Start culturing your own live algae or algae mixes for your cultures. Effective for; freshwater, marine or brackish, Rotifer, Copepods, Amphipods, or Spirulina.
- Culture Flask
- Culture Salts
- Culture Nutrients & Vitamins
- Algae Culture
- Culture Instructions
Nannochloropsis oculata, a single celled green algae.
Nannochloropsis oculata belongs to the class Eustigmatophyceae. This class consists of about six genera that were separated from the Xanthophyceae (Yellow-green algae); it seems likely that more general will be transferred into the class from the Xanthophyceae in the future. Used in the production of aquarium feeds, lipids for biofuels and nutraceuticals (derived from food),
The eustigmatophyceae are small microalgae that are planktonic or grow attached to various substrates. They are unique in lacking chlorophyll pigments other than Chlorella a (Fisher et al. 1998).
Nannochloropsis oculata is one of the five identified species of the genus. These species are characterized by small cells with a simple morphology and typically live in salt-water habitats. Nannochloropsis species are widely used as food in aquaculture and have been proposed as a commercial source for the dietary supplement a omega-3 fatty acid (Sukenik et al. 1989).
Nannochloropsis is a very useful genus of algae. It grows fast and produces large quantities of biomass. The cells of this species are small 2 micron (um), non-motile (not capable of movement), green in color. It is different from other related microalgae in that it lacks chlorophyll-n and c. Nannochloropsis is able to build up a high concentration of a range of pigments such as astaxanthin (a potent antioxidant) and canthaxanthin (a orange-yellow pigment).
Isochrysis galbana, a single celled golden algae.
Nearly every aquaculture facility that farms filter feeders will culture Isochrysis as a food supply for their farmed organisms. Isochrysis galbana, like other members of this class, is rich in polyunsaturated fatty acids including DHA, which are of nutritional value for marine fish larvae and juvenile stages of molluscs. This organism grows well in captivity and is a “model organism” commonly used by researchers who study marine systems and photosynthesis.
The thallus, or body of the organism, appears as a single cell with a flagella. This strain also has a high concentration of the pigment fucoxanthin which is an accessory pigment for photosynthesis, and some have claimed that it has nutraceuticals benefits for humans and animals for weight loss.
Growing Isochrysis requires seawater. As they are flagellated, they can grow without mixing being a requirement, however mixing does greatly help to increase growth the rates
Chlorella vulgaris, a single celled green algae.
One of the most studied genus of algae, Chlorella has been used to understand energy metabolism. This freshwater algae was employed in the 1940-1950’s by Otto Warburg in Germany to build an understanding of respiration and photosynthesis. His work was expanded upon by Melvan Calvin, James Bassham, and Andrew Benson, using radioactively labeled carbon (C14) at the university of California, Berkley. There work led to the understanding of carbon fixation (turning non biological carbon into new organic molecules). Many people believe Chlorella could serve as a potential source of food and energy because its photosynthetic efficiency is very high, compared to other crops such as sugar cane.
Chlorella is a freshwater genus of single-cell green algae belonging to the phylum Chlorphyta. Its is spherical in shape, about 2 to 10μm in diameter, and does not have a flagella. Chlorella contains the green photosynthetic pigments chlorophyll-a and -b in its chloroplast. Through photosynthesis, it multiplies rapidly, requiring only carbon dioxide, water, sunlight, and a small amount of minerals to reproduce.
Spirulina, Arthrospira platensis, Colonial cyanobacteria
Spirulina is the common name of the colonial blue-green algae Arthrospira platensis. Arthrospira occurs naturally worldwide and is cultivated on every continent except for Antarctica. It is farmed in large outdoor ponds, dried and sold into the dietary supplement, animal feed, and pigment markets. As a supplement, claims have been made regarding spirulina’s anti-inflammatory properties, believed to arise from the pharmacology of the pigment phycocyanin.
Spirulina contains more protein by mass than steak. As an animal feed, it supplies vitamins and can add pigmentation to fish. The pigment phycocyanin is useful for research and in the medical industry as it has unique fluorescent properties.
When starting a Arthrospira culture, it is important to keep the light level low for the first weeks, as the culture is getting used to its new habitat. Also, the water must be chlorine free, using bottled drinking water is best. For larger cultures, using a carbon filter to treat municipal water will often work just fine. Healthy Arthrospira is positively buoyant and will float to the top of the water mix it back into the water column with air bubbles to maximise growth rates.
Dunaliella salina – Orange/ Red
Dunaliella salina also contains super-oxide dismutase (SOD), lutein, cryptoxanthin and zeaxanthin which are antioxidants that can boost skin hydration and promote normal healthy skin. Dunaliella is a unique unicellular species of algae containing rich concentrations of carotenoids (mainly Beta-Carotene), antioxidants and essential vitamins. Among various carotenoid-rich microalgae, D. salina has the greatest carotene concentration making up ~10% algal dry weight. Dunaliella salina can reproduce asexually, sexually and through division of motile vegetative cells.
Tetraselmis chuii – Green
Thalassiosira pseudonan – diatom species from red to green.
Thalassiosira pseudonana is a marine centric diatom. Diatoms are unicellular, eukaryotic, phytoplankton that display a unique evolutionary history and provide major ecological contributions in marine environments. Diatoms are capable of photosynthesis, having acquired plastids through secondary endosymbiosis of primary endosymbionts, including plants and, green algae, red algae, and glaucophytes. Having evolved 91.5 million years ago during the Upper Turonian period , analyses of these organisms display long-term contributions to deposits of diatomite, carbon cycling, global climate, and petroleum reserves. Today diatoms continue to have major ecological implications by playing a fundamental role in global carbon cycling and global climate. As a result of the ecological importance of diatoms, T. pseudonana was the first diatom to undergo full genome sequencing. In addition, T. pseudonana have elaborate silicified cell wall nanostructures that may contribute to future study of silica nanotechnology. T. pseudonana diatoms display a unique combination of metabolic processes including genes for nitrogen fixation, the urea cycle, carbon fixation, iron uptake, and photosynthesis .
Haematococcus pluvialis – Green (freshwater and coastal brackish)
3 Major Health Benefits of Astaxanthin; Astaxanthin is known to powerfully enhance brain function, eye health, immunity and joint function among other things. The greatest natural source for astaxanthin is in the unique microalgae Haematococcus pluvialis. Safety of an antihistamine-rich Haematococcus pluvialis algal extract: a randomized clinical trial. A growing body of scientific literature indicates that astaxanthin is a more powerful antioxidant than other carotenoids and vitamin E and may confer numerous health benefits. The green algae H. pluvialis makes high amounts of astaxanthin when its condition is unfavorable, including high UV exposure, which is why astaxanthin has strong anti-oxidative properties (R).
Anabaena variabilis – Green (freshwater) cyanobacteria
Anabaena variabilis is a species of filamentous cyanobacteria. This species of the genus Anabaena and the domain Eubacteria is capable of photosynthesis. This species is also known to be heterotrophic in that it may grow without light in the presence of fructose. It also can convert atmospheric dinitrogen to ammonia via nitrogen fixation.
Anabaena variabilis is a phylogenic-cousin of the more well-known species Nostoc spirrilum. Both of these species along with many other cyanobacteria are known to form symbiotic relationships with plants. Other cyanobacteria are known to form symbiotic relationships with diatoms, though no such relationship has been observed with Anabaena variabilis.
Anabaena variabilis is also a model organism for studying the beginnings of multicellular life due to its filamentous characterization and cellular-differentiation capabilities.
Animals that eat these microalgae then absorb astaxanthin into their bodies, which is why wild shrimp, lobster, crab, and salmon have bright red-orange colors.